Validating microarray data using rt real time pcr
Approximately 80% of selected upregulated and downregulated genes identified by microarray analysis using linearly amplified RNA were confirmed by QRT-PCR using non-amplified m RNA as the starting template.
1,375 genes represented by both microarray platforms and spanning a wide dynamic range in gene expression levels, were selected for Taq Man Gene Expression Assay based real-time PCR validation.
Together with your c DNA, these products contain all of the enzymes, buffers, primers and probe necessary to perform SYBR Green real-time PCR analysis.
DNA microarrays are rapidly becoming a fundamental tool in discovery-based genomic and biomedical research.
The next-generation sequencing technologies are being rapidly applied in biological research.
Tens of millions of short sequences generated in a single experiment provide us enormous information on genome composition, genetic variants, gene expression levels and protein binding sites depending on the applications.